Results - Principles of Microscopy and Imaging
The course participants followed our published protocols (Beatty,
B., Mai, S. Squire, J. FISH: A Practical Approach. Oxford University
Press. 2002). They performed fluorescent in situ hybridization (FISH)
on mouse metaphase chromosomes.
Four groups of participants imaged at 4 different workstations
in the Genomic Centre for Cancer Research and Diagnosis www.umanitoba.ca/institutes/manitoba_institute_cell_biology
/GCCRD/Index3.htm
1. The centromeres are stained in yellow, the chromosomes in blue.
Each participant in each group acquired images. The best image has
been selected and is shown below.
(Fig. 1).
2. Another group of participants received mouse chromosome 11 paints
and performed FISH with those. Chromosome 11 is stained in red,
and all chromosomes are stained in blue. The best image that the
participants acquired was selected and is shown below.
(Fig. 2).
3. The course also introduced the participants to fluorescent immunohistochemistry.
Mouse lymphocytes were stained with anti-B220 (green) and anti-Max
(red) antibodies. A selected image taken by one of the groups is
shown below.
(Fig.3).
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